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Image Search Results
Journal: Advanced therapeutics
Article Title: Organ-restricted vascular delivery of nanoparticles for lung cancer therapy
doi: 10.1002/adtp.202000017
Figure Lengend Snippet: Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and CCR2 in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.
Article Snippet: The sections were incubated with Roti-Block for 1 h at room temperature, and then with the primary antibody at 4°C overnight; i.e ., EGFR (Abcam, ab52894) and
Techniques: Generated, Avidin-Biotin Assay, Transmission Assay, Immunohistochemical staining, Staining