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Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and <t>CCR2</t> in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.
Ccr2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccr2/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
ccr2 - by Bioz Stars, 2026-02
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Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and <t>CCR2</t> in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.
Nbp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and <t>CCR2</t> in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.
Anti Ccr2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ccr2 antibody/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
anti ccr2 antibody - by Bioz Stars, 2026-02
94/100 stars
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Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and <t>CCR2</t> in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.
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Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and CCR2 in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.

Journal: Advanced therapeutics

Article Title: Organ-restricted vascular delivery of nanoparticles for lung cancer therapy

doi: 10.1002/adtp.202000017

Figure Lengend Snippet: Synthesis and characterization of mesoporous silica nanoparticles for passive and active targeting. a) Delayed co-condensation process leads to different core (green, thiol groups) and shell (red, amino groups) functionalization of MSN-SHin-NH2,out. (i) MSNBiotin nanoparticles were generated by first transforming amino groups into carboxy groups, followed by EDC amidation with the pH-cleavable AK linker, and subsequent addition of covalently bound biotin. (ii) MSNAVI nanoparticles were generated by the addition of avidin to efficiently seal the mesopores after cargo loading and covalent attachment of the dyes at the thiol groups in the inner pore system. b) Functionalized nanoparticles (MSNx) with active targeting were generated by covalent attachment of specific ligands to the outer surface of MSNAVI for lung cancer. c) Transmission electron micrograph (TEM) of MSN-SHin-NH2,out. Scale bar = 50 nm. d) Nitrogen sorption isotherms of MSN-SHin-NH2,out (black), MSNBiotin (green) and MSNAVI (red) showing efficient sealing of the pores. e) Dynamic light scattering (DLS) of MSNAVI (red) in water showing size uniformity. f) Time-dependent pH-responsive release at pH 7 and pH 5. ** p = 0.0026, two-way ANOVA, Sidak’s multiple comparisons test comparing cargo release between pH 5 and pH 7 over time. Values given are an average of three independent experiments ± standard error of the mean g) Immunohistochemical staining representing complementary distribution of EGFR and CCR2 in a human non-small cell lung cancer (NCSLC) specimen. Scale bar = 50 μm. h) Peptide sequences for EGFR and CCR2 targeting ligands, i.e., GE11 and ECL1i, respectively. i) Dynamic light scattering (DLS) of MSNtEGFR (green) and MSNtCCR2 (black) in water showing size stability following functionalization.

Article Snippet: The sections were incubated with Roti-Block for 1 h at room temperature, and then with the primary antibody at 4°C overnight; i.e ., EGFR (Abcam, ab52894) and CCR2 (Novus Biologicals, NBP1- 48338).

Techniques: Generated, Avidin-Biotin Assay, Transmission Assay, Immunohistochemical staining, Staining